Optically ground and polished milled glass chambers are diamond etched with Double improved Neubauer ruling. A “V” slash on each plateau facilitates charging and reduces the possibility of overflow. Metal plating on the plateaus provides sharp bright line contrast for consistent cell counts. Triple dividing lines clearly show cells within the counting area. Ruled surface is 0.1mm below the cover glass. Fluid capacity is 0.1mm3/mm2 and 0.00025mm3/each grid square.